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| SPRINT (Single Particle Reconstruction In No Time) is a program which can automatically process micrographs, extract particle images, and compute 3D density map of icosahedral virus to its best possible resolution while (or after) cryoEM data acquisition is on-going at the same time. Running this program is very easy and requires minimum user input at the beginning. First, user needs to start the cryoEM data acquisition session, or all the cryoEM micrographs are already available on phage cluster. Then, user needs to create a project directory in phage cluster. Thirdly, in this directory, run the sprint program by typing "sprint". The program will ask a series of questions, you can answer one by one: | SPRINT (Single Particle Reconstruction In No Time) is a program which can automatically process micrographs, extract particle images, and compute 3D density map of icosahedral virus to its best possible resolution while (or after) cryoEM data acquisition is on-going at the same time. Running this program is very easy and requires minimum user input at the beginning. First, user needs to start the cryoEM data acquisition session, or all the cryoEM micrographs are already available on phage cluster. Then, user needs to create a project directory in phage cluster. Thirdly, in this directory, run the sprint program by typing "sprint". The program will ask a series of questions, you can answer one by one: | ||
| - | 1) please enter the dir where the dm3 or mrc data is | + | 1) please enter the dir where the micrographs are: |
| - | 2) this is legionon (mrc)(default) or manual data in dm3 (m) or pif (p)? | + | 2) this is leginon (mrc)(default) or manual data in dm3 (m) or pif (p)? |
| 3) please enter the pixel size | 3) please enter the pixel size | ||