Differences

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--- localfsc [2015/09/04 14:33]
gcardone [Installation]
+++ localfsc [2015/09/04 14:55]
gcardone [Usage]
@@ Line 21: / Line 21: @@
 ===== Usage =====
-A test data set is provided together with a tutorial which describes the procedure to get from the raw micrographs to a helical reconstruction.
+Once opened, the LocalFSC module, a help button provides all the basic information on how to use it and on all the different options available in the interface.
-[[http://cryoem.ucsd.edu/programs/IHRSR++tutorial_v1.5.pdf|Tutorial]] (pdf)
+In order to get familiar with the module, here is provided a [[http://cryoem.ucsd.edu/programs/code/TestCaseLFSC.tar.gz|Test Dataset]] to download, which contains a simulated reconstruction of a ribosome (see article below for details). Specifically, the archive file contains three maps (two half maps and one map from all the particles) and a Chimera marker file with two markers pointing to two domains with different occupancy level.
+  * Uncompress the data
-[[http://cryoem.ucsd.edu/programs/code/IHRSR_test_prob.tar.gz|Test Dataset]] (**~5 GB file**)
+  * Open the tree maps and the marker file in Chimera
--------------------------
+  * Verify that all three maps are overlapping and their pixel size is properly set in the Features->Coordinates Menu of the Volume Viewer dialog window.
+  * Hide the two half maps
+  * Open the LocalFSC
 ===== Credits =====