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localfsc [2015/09/04 14:33] gcardone [Installation] |
localfsc [2015/09/04 14:52] gcardone |
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| ===== Usage ===== | ===== Usage ===== | ||
| - | A test data set is provided together with a tutorial which describes the procedure to get from the raw micrographs to a helical reconstruction. | + | Once opened, the LocalFSC module, a help button provides all the basic information on how to use it and on all the different options available in the interface. |
| - | [[http://cryoem.ucsd.edu/programs/IHRSR++tutorial_v1.5.pdf|Tutorial]] (pdf) | + | In order to get familiar with the module, here is provided a [[http://cryoem.ucsd.edu/programs/code/TestCaseLFSC.tar.gz|Test Dataset]] to download, which contains a simulated reconstruction of a ribosome (see article below for details). Specifically, the archive file contains three maps (two half maps and one map from all the particles) and a Chimera marker file with two markers pointing to two domains with different occupancy level. |
| - | + | * Uncompress the data | |
| - | [[http://cryoem.ucsd.edu/programs/code/IHRSR_test_prob.tar.gz|Test Dataset]] (**~5 GB file**) | + | * Open the tree maps and the marker file in Chimera |
| + | * | ||
| ------------------------- | ------------------------- | ||