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localfsc [2015/09/04 14:27]
gcardone
localfsc [2015/09/04 14:52]
gcardone
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 ===== Installation ===== ===== Installation =====
  
-  *     If you don't already have a directory on your computer that is specific for external plugins for chimera, create ​a new one.+  *     If you don't already have a directory on your computer that is specific for external plugins for chimera, create one.
   *     ​Download the file [[http://​cryoem.ucsd.edu/​programs/​code/​LocalFSC_chimera.tar.gz|LocalFSC_chimera.tar.gz]] in this directory and uncompress it.   *     ​Download the file [[http://​cryoem.ucsd.edu/​programs/​code/​LocalFSC_chimera.tar.gz|LocalFSC_chimera.tar.gz]] in this directory and uncompress it.
   *     In Chimera, go to Favorites->​Preferences,​ from Category select Tools and add the directory containing the LocalFSC folder to the Locations.   *     In Chimera, go to Favorites->​Preferences,​ from Category select Tools and add the directory containing the LocalFSC folder to the Locations.
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 ===== Usage ===== ===== Usage =====
-A test data set is provided together with tutorial which describes ​the procedure ​to get from the raw micrographs to a helical reconstruction.+Once opened, the LocalFSC module, ​help button provides all the basic information on how to use it and on all the different options available in the interface.
  
-[[http://​cryoem.ucsd.edu/​programs/​IHRSR++tutorial_v1.5.pdf|Tutorial]] (pdf) +In order to get familiar with the module, here is provided a [[http://​cryoem.ucsd.edu/​programs/​code/​TestCaseLFSC.tar.gz|Test Dataset]] ​to download, which contains a simulated reconstruction of a ribosome ​(see article below for details). Specifically,​ the archive file contains three maps (two half maps and one map from all the particles) and a Chimera marker file with two markers pointing to two domains with different occupancy level. 
- +  ​Uncompress the data 
-[[http://​cryoem.ucsd.edu/​programs/​code/​IHRSR_test_prob.tar.gz|Test Dataset]] (**~5 GB file**)+  ​Open the tree maps and the marker ​file in Chimera 
 +  ​
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