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IHRSR++ is a user-friendly extension to Ed Egelman's Iterative Helical Real Space Reconstruction (IHRSR) software.

It includes all of the original IHRSR functionality plus the capabilities to determine out-of-plane tilt, apply dihedral symmetry, and automatically construct a starting model from the image data.

IHRSR++ also contains a number of Perl and Spider scripts for performing common preprocessing and data manipulation tasks such as power spectra calculations, application of CTF corrections, image stack generation, image centering, and tube diameter determination.

IHRSR++ is free software, licensed under the BSD license.

Disclaimer: Although we have successfully used it for a number of reconstructions, it should be considered a work in progress. Owing to time constraints, neither the Egelman nor Baker labs are able to provide support. The software is provided purely as a convenience to those attempting helical reconstructions.