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auto3dem:userguide [2016/01/13 14:12]
gcardone [po2r]
auto3dem:userguide [2016/01/19 13:02]
admin [Auto3dem keyword default values]
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 **freeze_res**:​ non-zero value freezes the resolutions used in PPFT, and PO2R (resolution used in P3DR will still be based on the results from PSF). **freeze_res**:​ non-zero value freezes the resolutions used in PPFT, and PO2R (resolution used in P3DR will still be based on the results from PSF).
  
-**fsc_hithresh**: cutoff value for FSC used in estimating map resolution.+**fsc_cutoff**: cutoff value for FSC used in estimating map resolution. If not specified it is set either to 0.143 (gold_standard set to 1) or to 0.5 (gold_standard set to 0). 
 + 
 +**fsc_hithresh**:​ cutoff value for FSC used in filtering the map.
  
 **fsc_lothresh**:​ cutoff value for FSC used to set resolution limits in P3DR, PSF, and PO2R. **fsc_lothresh**:​ cutoff value for FSC used to set resolution limits in P3DR, PSF, and PO2R.
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 **magref_reset**:​ 1 to ‘forget’ magnification assigned to  data, 0 to refine around the current estimated value. **magref_reset**:​ 1 to ‘forget’ magnification assigned to  data, 0 to refine around the current estimated value.
  
-**magref_step**:​ size of magnification refinement step (microns).+**magref_step**:​ size of magnification refinement step (fraction).
  
 **mask_map**:​ name of binary map to use for focused refinement. Only used for modes //local// and //​ticos_equiv//​. Default is ‘none’, a reserved string indicating that no mask is used. **mask_map**:​ name of binary map to use for focused refinement. Only used for modes //local// and //​ticos_equiv//​. Default is ‘none’, a reserved string indicating that no mask is used.
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 <WRAP center round box 75%> <WRAP center round box 75%>
 ''​local'':​ local refinement (//nangle// steps of //dangle// degrees along each direction)\\ ''​local'':​ local refinement (//nangle// steps of //dangle// degrees along each direction)\\
-''​mag'':​ magnification refinement (//​nmagref//​ steps of //​magref//​_step ​microns ​along each direction)\\+''​mag'':​ magnification refinement (//​nmagref//​ steps of //​magref//​_step ​fraction ​along each direction)\\
 ''​global'':​ global search in one asymmetric unit (on a grid with step of //gangle// degrees)\\ ''​global'':​ global search in one asymmetric unit (on a grid with step of //gangle// degrees)\\
 ''​ticos_equiv'':​ restricted search to the 60 symmetry related orientations. ''​ticos_equiv'':​ restricted search to the 60 symmetry related orientations.
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 | auto | freeze_annulus | 0 | | auto | freeze_annulus | 0 |
 | auto | freeze_res | 0 | | auto | freeze_res | 0 |
 +| auto | fsc_cutoff | 0.143 if gold_standard=1,​ 0.5 otherwise |
 | auto | fsc_hithresh | 0.5 | | auto | fsc_hithresh | 0.5 |
 | auto | fsc_lothresh | 0.3 | | auto | fsc_lothresh | 0.3 |