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Auto3DEM

Auto3DEM is a semi-automated image reconstruction system that provides all the tools to process raw micrographs acquired at an electron microscope and generate a three-dimensional reconstruction. The most intensive computational procedure like orientation search and reconstruction can be executed either in serial or in parallel on a computer cluster. A graphical interface, RobEM, is also provided both for image processing and visualization purposes.


Documentation


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Tutorials


Citations

To reference this program, please cit:e

Yan X., R. S. Sinkovits, and T. S. Baker (2007) AUTO3DEM - an automated and high throughput program for image reconstruction of icosahedral particles. J. Struct. Bio. 157:73-82. (pdf)

If you generate an initial model using the Random Model Computation method, please also cite:

Yan X., K. A. Dryden, J. Tang, and T. S. Baker (2007) Ab initio random model method facilitates 3D reconstruction of icosahedral particles. J. Struct. Bio. 157:211-225. (pdf)


License

AUTO3DEM is free to use for educational, research and non-profit purposes (see LICENSE file).


Funding


Credits

Tim Baker, Dan Marinescu, James Conway, David Belnap, Robert Sinkovits, Fuller, Giovanni Cardone, Xiaodong Yan, Benes Trus, Rob Ashmore