Transmission Electron Microscopy
Spot Scanning

Index

Suggested Reading

Extensive List

Meeting Abstracts, Newsletters and Bulletins

Suggested Reading

Downing, K. H. and R. Glaeser (1986) Improvement in high resolution image quality of radiation-sensitve specimens achieved with reduced spot size of the electron beam. Ultramicrosc. 20:269-278.

Bullough, P. and R. Henderson (1987) Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens. Ultramicrosc. 21:223-230.

Downing, K. H. (1991) Spot-scan imaging in transmission electron microscopy. Science 251:53-59.

Brink, J., W. Chiu and M. Dougherty (1992) Computer-controlled spot-scan imaging of crotoxin complex crystals with 400 keV electrons at near-atomic resolution. Ultramicrosc. 46:229-240.

Downing, K. H. (1992) Automatic focus correction for spot-scan imaging of tilted specimens. Ultramicrosc. 46:199-206.


Extensive List

Downing, K. H. and R. Glaeser (1986) Improvement in high resolution image quality of radiation-sensitve specimens achieved with reduced spot size of the electron beam. Ultramicrosc. 20:269-278.

Bullough, P. and R. Henderson (1987) Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens. Ultramicrosc. 21:223-230.

Downing, K. H. (1988) Observation of restricted beam-induced specimen motion with small-spot illumination. Ultramicrosc. 24:387-398.

Zemlin, F. (1989) Dynamic focusing for recording images from tilted samples in small-spot scanning with a transmission electron microscope. J. Elec. Microsc. Tech. 11:251-257.

Bullough, P. A. (1990) Imaging of protein molecules-towards atomic resolution. Elect. Microsc. Rev. 3:249-267.

Bullough, P. A. and P. A. Tulloch (1990) High-resolution spot-scan electron microscopy of microcrystals of an alpha-helical coiled-coil protein. J. Mol. Biol. 215:161-173.

Henderson, R., J. M. Baldwin, T. A. Ceska, F. Zemlin, E. Beckmann and K. H. Downing (1990) Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213:899-929.

Hernandez, F., H. Akahori, Y. Hosaka, N. Ikegami and K. Akatani (1990) The finest ultrastructural details of the inner layer of rotavirus revealed by mininimal electron doses. J. Elec. Microsc. 39:105-107.

Jap, B. K., K. H. Downing and P. J. Wallian (1990) Structure of PhoE porin in projection at 3.5 angstroms resolution. J. Struct. Biol. 103:57-63.

Downing, K. H. (1991) Spot-scan imaging in transmission electron microscopy. Science 251:53-59.

Brink, J. and W. Chiu (1991) Contrast analysis of cryo-images in n-paraffin recorded at 400kV out to 2.1 angstroms resolution. J. Microsc. 161:279-295.

Bullough, P. A. and P. A. Tulloch (1991) Spot-scan imaging of microcrystals of an influenza neuraminidase-antibody fragment complex. Ultramicrosc. 35:131-143.

Cyrklaff, M., I. Tews, S. D. Fuller and W. Kuhlbrandt (1991) High resolution of non-crystalline specimens: Cryo-electron microscopy of adenovirus. Micron 22:31-32.

Jap, B. K., P. J. Walian and K. Gehring (1991) Structural architecture of an outer membrane channel as determined by electron crystallography. Nature 350:167-169.

Brink, J., W. Chiu and M. Dougherty (1992) Computer-controlled spot-scan imaging of crotoxin complex crystals with 400 keV electrons at near-atomic resolution. Ultramicrosc. 46:229-240.

Downing, K. H. (1992) Automatic focus correction for spot-scan imaging of tilted specimens. Ultramicrosc. 46:199-206.

Downing, K. H., A. J. Koster and D. Typke (1992) Overview of computer-aided electron microscopy. Ultramicrosc. 46:189-197.

Henderson, R. (1992) Image contrast in high-resolution electron microscopy of biological macromolecules: TMV in ice. Ultramicrosc. 46:1-18.

Koster, A. J., H. Chen, J. W. Sedat and D. A. Agard (1992) Automated microscopy for electron tomography. Ultramicrosc. 46:207-227.

Avila-Sakar, A. J., M. F. Schmid, L.-S. Li, F. G. Whitby, G. N. P. Jr and W. Chiu (1993) Preliminary electron crystallographic analysis of ice-embedded tropomyosin crystals. J. Struct. Biol. 110:67-74.

Schmid, M. F., J. Jakana, P. Matsudaira and W. Chiu (1993) Imaging frozen, hydrated acrosomal bundle from limulus sperm at 7Å resolution with a 400kV electron cryomicroscope. J. Mol. Biol. 230:384-386.

Soejima, T., M. B. Sherman, M. F. Schmid and W. Chiu (1993) 4Å projection map of bacteriophage T4 DNA helix- destabilizing protein (gp32*I) crystal by 400-kV electron cryomicroscopy. J. Struct. Biol. 111:9-16.

Wolf, S. G., G. Mosser and K. H. Downing (1993) Tubulin conformation in zinc-induced sheets and macrotubes. J. Struct. Biol. 111:190-199.

Zhou, Z. H. and W. Chiu (1993) Prospects of using an IVEM with a FEG for imaging macromolecules toward atomic resolution. Ultramicrosc. 49:407-416.

Zhou, Z. H., B. V. V. Prasad, J. Jakana, F. J. Rixon and W. Chiu (1994) Protein subunit structures in the herpes simplex virus A-capsid determined from 400kV spot-scan electron cryomicroscopy. J. Mol. Biol. 242:456-469.

Thuman-Commike, P. A. and W. Chiu (1995) Automatic detection of spherical particles from spot- scan electron microscopy images. J. Microsc. Soc. Am. 1:191-201.

Zhou, Z. H., J. He, J. Jakana, J. D. Tatman, F. J. Rixon and W. Chiu (1995) Assembly of VP26 in Herpes simplex virus-1 capsid implicated by 3D structure of recombinant capsid. JMSA Proc. Microsc. Microanal. 53:840-841.

Thuman-Commike, P. A. and W. Chiu (1996) PTOOL: A software package for the selection of particles from electron cryomicroscopy spot-scan images. J. Struc. Biol. 116:41-47.

Meeting Abstracts, Newsletters and Bulletins

Downing, K. H. (1987) Overcoming beam-induced specimen motion: A major advance in improving image contrast for organic specimens. Elec. Microsc. Soc. Am. Proc. 45:6-9.

Brink, J. and W. Chiu (1990). Analysis of image contrast in high-resolution images of n-paraffin recorded at 400 kV and -167_C. Proc. XII Int'l. Cong. Elec. Microsc. (Seattle) 1:86-87.

deJong, M. J. C. and D. Typke (1990). Dynamic focussing of tilted speciments in the CM TEM via remote control. Proc. XII Int'l. Cong. Elec. Microsc. (Seattle) 1:150-151.

Downing, K. H. (1990). Spot scan imaging and the future of high resolution. Proc. XII Int'l. Cong. Elec. Microsc. (Seattle) 1:88-89.

Kuhlbrandt, W., D. N. Wang and K. H. Downing (1990). High-resolution electron crystallography of the light- harvesting chlorophyll-a/b protein complex from chloroplast membranes. Proc. XII Int'l. Cong. Elec. Microsc. (Seattle) 1:610.

Typke, D., G. Pfeifer, R. Hegerl and W. Baumeister (1990). 3D reconstruction of single particles by quasi-conical tilting from micrographs recorded with dynamic focusing. Proc. XII Int'l. Cong. Elec. Microsc. (Seattle) 1:244-245.

Downing, K. H. (1991). Instrumentation and methodology for electron crystallography. Proc. Elect. Microsc. Soc. Am. 49:420-421.

Fan, G. Y., A. J. Gubbens, O. L. Krivanek, M. L. Leber and P. E. Mooney (1991). Combining slow-scan CCD images to extend image size and dynamic range. Proc. Elect. Microsc. Soc. Am. 49:524-525.

deJong, M. J. C., W. M. Musing and M. T. Otten (1992) Enhanced information from biological materials through technological improvements in cryo-electron microscopy. Proc. Elect. Microsc. Soc. Am. 50:788-789.

Jakana, J., M. F. Schmid, P. Matsudaria and W. Chiu (1992) High resolution spot scan imaging of frozen, hydrated actin bundles with 400kV electrons. Elec. Microsc. Soc. Am. Proc. 50:512-513.


Top of page
Last edited April 29, 1997