Transmission Electron Microscopy
Radiation and Other Damage in Biological Specimens

Index

Cryo-microscopy Specimens - Suggested List

Other Specimens - Suggested List

Cryo-microscopy Specimens - Extensive List

Meeting Abstracts, Newsletters and Bulletins Concerning Cryo-microscopy Specimens

Other Specimens

Suggested List

Cryo-microscopy Specimens

Glaeser, R. M. and K. A. Taylor (1978) Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: A review. J. Microsc. 112:127-138.

Newmark, P. (1982) Cryo-transmission microscopy: fading hopes. Nature 299:386-387.

Lepault, J., J. Dubochet, I. Dietrich, E. Knapek and E. Zeitler (1983) Amendment to: Electron beam damage to organic specimens at liquid helium temperature. J. Mol. Biol. 163:511.

Conway, J. F., B. L. Trus, F. P. Booy, W. W. Newcomb, J. C. Brown and A. C. Steven (1993) The effects of radiation damage on the structure of frozen hydrated HSV-1 capsids. J. Struc. Biol. 111:222-233.


Other Specimens

Glaeser, R. M. (1971) Limitations to significant information in biological electron microscopy as a result of radiation damage. J. Ultrastruc. Res. 36:466-482.

Glaeser, R. M. (1975) Radiation damage and biological electron microscopy. In Physical Aspects of Electron Microscopy and Microbeam Analysis (B. Siegel and D. R. Beaman, eds.) John Wiley and Sons, N. Y. pp. 205-229.

Cosslett, V. E. (1978) Radiation damage in the high resolution electron microscopy of biological materials: A review. J. Microsc. 113:113-129.


Extensive List

Cryo-microscopy Specimens

Glaeser, R. M. and K. A. Taylor (1978) Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: A review. J. Microsc. 112:127-138.

Hayward, S. B. and R. M. Glaeser (1979) Radiation damage of purple membrane at low temperature. Ultramicrosc. 4:201-210.

Dietrich, I., H. Formanek, F. Fox, E. Knapek and R. Weyl (1979) Reduction of radiation damage in an electron microscope with a superconducting lens system. Nature 277:380-381.

Talmon, Y., H. T. Davis, L. E. Scriven and E. L. Thomas (1979) Mass loss and etching of frozen hydrated specimens. J. Microsc. 117:321-332.

Hayward, S. B. and R. M. Glaeser (1980). Use of low temperatures for electron diffraction and imaging of biological macromolecular arrays. Electron Microscopy at Molecular Dimensions. (Eds., W.Baumeister & W. Vogell) Springer-Verlag, Berlin. pp. 226-233.

Knapek, E. and J. Dubochet (1980) Beam damage to organic material is considerably reduced in cryo-electron microscopy. J. Mol. Biol. 141:147-161.

Lepault, J. and J. Dubochet (1980) Freezing, fracturing, and etching artifacts in particulate suspensions. J. Ultrast. Res. 72:223-233.

Chiu, W., E. Knapek, T. W. Jeng and I. Dietrich (1981) Electron radiation damage of a thin protein crystal at 4_K. Ultramicrosc. 6:291-296.

Dubochet, J., E. Knapek and I. Dietrich (1981) Reduction of beam damage by cryoprotection at 4_K. Ultramicroscopy 6:77-80.

Chiu, W. (1982). High-resolution electron microscopy of unstained, hydrated protein crystals. Electron Microscopy of Proteins. (Ed. J. R. Harris) Academic Press, N. Y. pp. 233-259.

Dubochet, J., J. Lepault, R. Freeman, J. A. Berriman, Homo and J.-C. (1982) Electron microscopy of frozen water and aqueous solutions. J. Microsc. 128:219-237.

Dubochet, J., J.-J. Chang, R. Freeman, J. Lepault, and A. W. McDowall (1982) Frozen aqueous suspensions. Ultramicrosc. 10:55-62.

Eusemann, R., H. Rose and J. Dubochet (1982) Electron scattering in ice and organic materials. J. Microsc. 128:239-249.

Newmark, P. (1982) Cryo-transmission microscopy: fading hopes. Nature 299:386-387.

Talmon, Y. (1982) Thermal and radiation damage to frozen hydrated specimens. J. Microsc. 125:227-237.

Lepault, J., J. Dubochet, I. Dietrich, E. Knapek and E. Zeitler (1983) Amendment to: Electron beam damage to organic specimens at liquid helium temperature. J. Molec. Biol. 163:511.

Lepault, J., R. Freeman and J. Dubochet (1983) Electron beam induced 'vitrified ice'. J. Microsc. 132:RP3-RP4.

Talmon, Y. (1984) Radiation damage to organic inclusions in ice. Ultramicrosc. 14:305-316.

Downing, K. H. and Glaeser, R. M. (1986) Improvement in high resolution image quality of radiation-sensitive specimens achieved with reduced spot size of the electron beam. Ultramicrosc. 20:269-278.

Talmon, Y., M. Adrian and J. Dubochet (1986) Electron beam damage to organic inclusions in vitreous, cubic, and hexagonal ice. J. Microsc. 141:375-384.

Lamvik, M. K., D. A. Kopf and S. D. Davilla (1987) Mass loss rate in collodion is greatly reduced at liquid helium temperature. J. Microsc. 148:211-217.

Dubochet, J., M. Adrian, J.-J. Chang, J.-C. Homo, Lepault, J., A. W. McDowall and P. Schultz (1988) Cryo-electron microscopy of vitrified specimens. Quart. Rev. Biophys. 21:129-228.

Downing, K. H. (1988) Observations of restricted beam-induced specimen motion with small-spot illumination. Ultramicrosc. 24:387-398.

Henderson, R. (1990) Cryo-protection of protein crystals against radiation damage in electron and x-ray diffraction. Proc. Roy. Soc. Lond. Series B: Biological Sciences 241:6-8.

Lamvik, M. K. (1991) Radiation damage in dry and frozen hydrated organic material. J. Microsc. 161:171-181.

Conway, J. F., B. L. Trus, F. P. Booy, W. W. Newcomb, J. C. Brown and A. C. Steven (1993) The effects of radiation damage on the structure of frozen hydrated HSV-1 capsids. J. Struc. Biol. 111:222-233.

Frederik, P. M., P. H. H. Bomans and M. C. A. Stuart (1993) Matrix effects and the induction of mass loss or bubbling by the electron beam in vitrified hydrated specimens. Ultramicrosc. 48:107-119.

Meeting Abstracts, Newsletters and Bulletins Concerning Cryo-microscopy Specimens

Glaeser, R. M. and S. B. Hayward (1978) Measurement and reduction of damage in frozen hydrated crystalline specimens. Proc. Ninth Int'l. Cong. Elec. Microsc. (Toronto) 2:10-11.

Glaeser, R. M. and S. B. Hayward (1978) Measurement and reduction of radiation damage in frozen hydrated crystalline specimens. Proc. Ninth Int'l. Cong. Elec. Microsc. (Toronto) 3:70-77.

Dubochet, J., A. McDowall, R. Freeman and J. Lepault (1982) Cryoprotection on organic specimens. Proc. Tenth Int'l. Cong. Elec. Microsc. (Hamburg) 1:19-23.

Conway, J. F., B. L. Trus, F. P. Booy, W. W. Newcomb, J. C. Brown and A. C. Steven (1992) Effects of Radiation Damage on Frozen Hydrated Capsids of HSV-1. Proc. Elect. Microsc. Soc. Am. 50:532-533.


Other Specimens

Hillier, J., S. Mudd, A. G. Smith and E. H. Beutner (1950) The 'fixation' of electron microscopic specimens by the electron beam. J. Bact. 60:641-654.

Bahr, G. F., F. B. Johnson and E. Zeitler (1965) The elementary composition of organic objects after electron irradiation. Lab. Invest. 14:377-395.

Stenn, K. and G. F. Bahr (1970) Specimen damage caused by the beam of the transmission electron microscope, a correlative reconsideration. J. Ultrastruc. Res. 31:526-550.

Williams, R. C. and H. W. Fisher (1970) Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposure. J. Mol. Biol. 52:121-123.

Glaeser, R. M. (1971) Limitations to significant information in biological electron microscopy as a result of radiation damage. J. Ultrastruc. Res. 36:466-482.

Thach, R. E. and S. S. Thach (1971) Damage to biological samples caused by the electron beam during electron microscopy. Biophys. J. 11:204-210.

Isaacson, M., D. Johnson and A. V. Crewe (1973) Electron beam excitation and damage of biological molecules; its implications for specimen damage in electron microscopy. Rad. Res. 55:205-224.

Unwin, P. N. T. (1974) Electron microscopy of the stacked disk aggregate of tobacco mosaic virus protein II. The influence of electron irradiation on the stain distribution. J. Mol. Biol. 87:657-670.

Dubochet, J. (1975) Carbon loss during irradiation of T4 bacteriophages and E. coli bacteria in electron microscopes. J. Ultrastruc. Res. 52:276-288.

Glaeser, R. M. (1975) Radiation damage and biological electron microscopy. In Physical Aspects of Electron Microscopy and Microbeam Analysis (B. Siegel and D. R. Beaman, eds.) John Wiley and Sons, N. Y. pp. 205-229.

Ottensmeyer, F. P., R. F. Whiting, E. E. Schmidt and R. S. Clemens (1975) Electron microtephroscopy of proteins: A close look at the ashes of myokinase and protamine. J. Ultrastruc. Res. 52:193-201

Salih, S. M. and V. E. Cosslett (1975) Radiation damage in electron microscopy of organic materials: Effect of low temperatures. J. Microsc. 105:269-276.

Hahn, M., J. Seredynski and W. Baumeister (1976) Inactivation of catalase monolayers by irradiation with 100keV electrons. Proc. Nat. Acad. Sci. (U.S.A.) 73:823-827.

Cosslett, V. E. (1978) Radiation damage in the high resolution electron microscopy of biological materials: A review. J. Microsc. 113:113-129.

Dietrich, I., J. Dubochet, F. Fox, E. Knapek and R. Weyl (1980) Reduction of radiation damage by imaging with a superconducting lens system. In Electron Microscopy at Molecular Dimensions (W. Baumeister and W. Vogell, eds.) Springer-Verlag, Berlin pp.234-244.

Fujiyoshi, Y., T. Kobayashi, K. Ishizuka, N. Uyeda, Y. Ishida and Y. Harada (1980) A new method for optimal-resolution electron microscopy of radiation-sensitive specimens. Ultramicrosc. 5:459-468.

Herrmann, K.-H., D. Krahl and H.-P. Rust (1980) Low-dose image recording by TV techniques. In Electron Microscopy at Molecular Dimensions (W. Baumeister and W. Vogell, eds.) Springer-Verlag, Berlin pp.186-193.

Kessel, M., J. Frank and W. Goldfarb (1980) Low-dose electron microscopy of individual biological macromolecules. In Electron Microscopy at Molecular Dimensions (W. Baumeister and W. Vogell, eds.) Springer-Verlag, Berlin pp.154-160.

Chiu, W. and T. W. Jeng (1982) Electron radiation sensitivity of protein crystals. Ultramicrosc. 10:63-70.

Hosoi, J. and T. Matsuo (1982) Radiation-induced changes in the images of a negatively stained specimen. Ultramicrosc. 7:233-240.

Downing, K. H. (1983) Temperature dependence of the critical electron exposure for hydrocarbon monolayers. Ultramicrosc. 11:229-238.

Wrigley, N. G., E. Brown and R. K. Chillingworth (1983) Combining accurate defocus with low-dose imaging in high resolution electron microscopy of biological material. J. Microsc. 130:225-232.

Jeng, T. -W. W. and Chiu (1984) Quantitative assessment of radiation damage in a thin protein crystal. J. Microsc. 136:35-44.

Henderson, R. and R. M.Glaeser (1985) Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals. Ultramicrosc. 16:139-150.

Berriman, J. and K. R. Leonard (1986) Methods for specimen thickness determination in electron microscopy. II. Changes in thickness with dose. Ultramicrosc. 19:349-366.

Bullough, P. and R. Henderson (1987) Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens. Ultramicrosc. 21(3):223-230.

Cermola, M. and W. -H. Schreil (1987) Size changes of polystyrene latex particles in the electron microscope under controlled physical conditions. J. Elec. Microsc. Tech. 5:171-179.

Dorset, D. L. and F. Zemlin (1987) Specimen movement in electron-irradiated paraffin crystals - A model for initial beam damage. Ultramicrosc. 21:263-270.

Jesior, J.-C. and R. H. Wade (1987) Electron-irradiation-induced flattening of negatively stained 2D protein crystals. Ultramicrosc. 21:313-320.

Luther, P. K., M. C. Lawrence, and R. A. Crowther (1988) A method for monitoring the collapse of plastic sections as a function of electron dose. Ultramicrosc. 24:7-18.



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Last edited April 29, 1997